MIMOLETTE LAB RAT

ENDOTRACHEAL INTUBATION

Place “Mimolette” supine on an intubation work stand.

Secure the rat mannikin to the stand by the upper incisors using an incisor loop.

Tilt the stand to a 45 degree angle.

Prepare the endotracheal tube over a guide wire. We recommend using a clear 14 guage catheter for Mimolette. Check that the catheter is loosely fitted onto the guide wire for easy removal.

Withdraw the tongue from the mouth and position it to the side of the mouth.

Insert the guide wire slowly and gently into the chords of the rat mannikin and into the trachea. Ensure it does not extend past the thoracic inlet.

To check that intubation is performed correctly on Mimolette, use the squeeze bulb provided in your kit and insert the tip of the bulb into the opening of the catheter. Gently squeeze one or two puffs of air into the rat mannikin. The thorax should rise and fall with the air being introduced into the lung.

* NOTE: Do not hook up Mimolette to the blood source during Intubation.

* NOTE: Please be aware that when transferring the skills learned on this model to a live rat, a smaller bulb must be used. The bulb provided in this kit is solely for the use on the Mimolette mannikin and must not be used on live rats as there is a danger of overinflating the lungs of a live rat with the bulb provided.

BLOOD COLLECTION BY CARDIAC PUNCTURE

Fill the blood bag with artificial blood and ensure the blood bag tube is clamped with the attached clamp.

Hang the blood bag from the IV stand provided in your kit.

Connect the blood bag tube to the tube on the shoulder of the rat mannikin securely.

Ensure the flow regulator on the tube at the leg of the rat mannikin is positioned “closed”.

Place the tube at the leg into a container to collect the blood flowing out.

Lay your rat mannikin flat or with it’s upper body slightly inclined.

Unclamp the tube on the blood bag to allow the artificial blood to flow through. Turn the flow regulator dial open to allow the artificial blood will run through the rat mannikin.

We recommend using a 25 gauge needle and 3 ml syringe.

Locate the position of the heart. On Mimolette, this position is about 5mm from the center, at the level of its axillary region.

Location 1:

Insert the needle about 10mm deep at an angle of about 30 degrees from the body in the direction towards the anterior.

Withdraw the artificial blood slowly as you would with a live rat. (Withdrawing the blood too quickly will kill a live rat!)

Location 2:

On the left side of the mannikin body, insert the needle at an angle 15 degrees towards the medial, in the pectoral region.

Withdraw the artificial blood slowly as you would with a live rat. (Withdrawing the blood too quickly will kill a live rat!)

BLOOD COLLECTION FROM THE SAPHENOUS VEIN

Fill blood bag with the artificial blood provided.

As with the procedure carried out for cardiac puncture: connect blood bag tube to the tube at the left shoulder of the rat.

Let blood run through the system and control with flow regulator. Let overflowing blood into a container.

Grasp or slightly stretch open the left thigh.

A tourniquet may be placed around the knee, but is not necessary.

Puncture the vein with a recommended 28 gauge lancet.

Collect drops of blood as they appear. You may need to control the amount of blood flowing through with the flow regulator.

Massage the thigh gently to increase collection.

* NOTE: Please do not attempt to puncture the mannikin in any other areas other than the heart locations (as described above) and the left saphenous vein. Puncturing the mannikin in any other point will cause damage to the mannikin.

CLEANING AND AFTER-CARE

When collection is complete, ensure the blood flow is completely closed off.

Wipe off any artificial blood from the mannikin.

Remove the blood from the blood bag and fill with clean water.

Run clean water through the mannikin.

Dry mannikin.

Using a Q-tip, dab punctured area with special sealant provided in your kit and let dry for at least 20 minutes.

Repeat after every puncture, especially with blood collection from the saphenous vein.

Over time, after many layers of sealant are applied, simply peel it off for a cleaner finish.